Cambodia is widely recognized as the most cost-effective country for in vitro fertilization (IVF) in Asia. Its IVF technology has reached an international advanced level, and its medical technology is closely following that of developed countries in Europe and America. This is attributed to Cambodia's early start in the research of IVF technology and its boasting of the world's most experienced reproductive medicine experts and top-notch embryo laboratories. With the in-depth research on in vitro fertilization (IVF), its process has been continuously improved.
Scientific Process
① Pre-test tube examination
Ask the client to undergo a pre-test tube examination. For women, the main examinations include AMH, six chromosomal eugenics tests, and determination of the size and shape of basal follicles in the ovaries. For men, the main examination is semen (abstinence for 3 to 7 days). If the patient has a special disease, a detailed examination report of the relevant disease must be provided to offer a basis for the doctor to understand the patient's physical condition.
② Ovulation induction
During each menstrual cycle, a woman releases multiple basal follicles, but only one matures. In an in vitro fertilization (IVF) assisted pregnancy cycle, it is necessary to obtain multiple mature eggs to increase the success rate. Therefore, ovulation induction drugs are needed to promote the simultaneous development and maturation of multiple follicles.
③ Egg retrieval
When 1 to 2 follicular diameters have developed > 18mm, 2 to 3 follicle diameters developing > When the egg size reaches 17mm, egg retrieval can be arranged.
The egg retrieval surgery is painless throughout. Under B-ultrasound monitoring, the doctor obtained the follicular fluid through the vagina with a fine puncture needle. In Cambodia, the egg retrieval surgery is performed under general anesthesia and the entire process only takes about 10 minutes. The follicular fluid drawn from each follicle is collected in a test tube, and each follicular fluid contains an egg. Subsequently, the doctor sent these test tubes, which carry the hope of life, into the laboratory. Under a microscope, the eggs were found and placed in special culture dishes. The culture medium in these culture dishes was made to simulate the fluid of the fallopian tubes, so the eggs could survive safely.
④ Sperm collection
For a normal male, sperm collection only requires stimulating the sexual organs to achieve ejaculation, and then the semen needs to be washed. If a man has azoospermia due to vas deferens obstruction, he can use testicular puncture technology to search for healthy sperm in the testicles, epididymis or seminiferous tubules.
⑤ Fertilization process
Cambodian experts have chosen ICSI, or intracytoplasmic sperm injection, to screen for the healthiest sperm to combine with the egg. They use a microscopic operating system to inject a single sperm into the egg to increase the fertilization rate of the egg.
⑥ Blastocyst culture
In vitro fertilization (IVF) assisted pregnancy technology simulates the mother's environment, which better meets the growth needs of embryos. After fertilization, the egg forms a fertilized egg, which divides into two cells on the first day. The next day, it will divide into a solid cell mass of 4 to 6 cells. On the third day, it will divide into an early embryo with 8 to 12 cells. On the fourth day, the speed of cell division increases. By the fifth day, the fertilized egg will develop into a blastocyst composed of over a hundred cells.
⑦ Cell Extraction
Cell extraction is a key technology for genetic screening and diagnosis of blastocyst cells in the third-generation in vitro fertilization. The extracted cells are the parts that will develop into the placenta in the future and will not affect the quality of the blastocyst itself。
⑧ Genetic Screening
Genetic screening technology is used to detect the number and structural abnormalities of the 23 pairs of chromosomes in blastocysts. Through detection and comparison, it analyzes whether there are chromosomal abnormalities in the blastocyst. PGD genetic diagnosis technology diagnoses blastocyst cells to determine whether there are chromosomal abnormalities and genetic defect genes in the blastocyst. The combination of PGS genetic screening and PGD genetic diagnosis technology can avoid chromosomal abnormalities and hereditary diseases in families, and improve the success rate of in vitro fertilization.
⑨ Blastocyst transfer
The entire process of blastocyst transfer is monitored by B-ultrasound, and a very thin and soft tube is used to implant the selected healthy blastocysts into the uterus. The number of embryos to be transplanted can be determined based on the guest's needs and physical condition. The remaining blastocysts can be cryopreserved.
Personalized reproductive privileges
Legal gender selection + choosing multiple or boy-girl twins + LGBT, single, HIV and other groups can have children through third-party assisted reproduction
